| Congresso Brasileiro de Microbiologia 2023 | Resumo: 849-1 | ||||
Resumo:Salmonellosis is an important gastrointestinal infection in humans and cause of foodborne outbreaks in the world. In this context, molecular characterization is essential to understand how the strains circulate. The aim of this study was to evaluate the genotypic distribution of S. Heidelberg according to the source of isolation. The genetic relatedness of the S. Heidelberg isolates was determined by pulsed-field gel electrophoresis (PFGE). Isolates were received in The National Reference Laboratory of Cholera and Enteric Diseases of the Oswaldo Cruz Institute Foundation - FIOCRUZ, Rio de Janeiro, Brazil (NRL) inoculated in nutrient agar and on arrival, confirmed the identification as S. enterica by serotyping according to the KauffmanWhite scheme. NRL receives Salmonella strains from Brazilian meat industries, health services laboratories and universities for serotyping and genotyping. Strains isolated from the 1990s to 2017 obtained from different sources and regional origins were included. The most prevalent pulsotypes of cluster A were BRJF6X01.006 (27/95 = 28,42%) related between 1995 and 2011 in broilers, poultry meat and poultry farms, meat product and human, and BRJF6X01.001 (21/95 = 22,10%) related between 2011 and 2017 in wild animals, broilers, poultry meat, poultry farms, meat product, animal feed, and pork meat. The pulsotype BRJF6X01.001 shows a high distribution in the environmental and productive chain. The degree of similarity between pulsotypes BRJF6X01.006 and BRJF6X01.001 is 88%. To ensure the safety of human and animal health, holistic approaches, including surveillance of Salmonella throughout the environment and in the production chain, together with control measures, are critical. As transmission of Salmonella from food producing animals to wildlife and to the environment is considered potential public health problem, information on the survival and persistence of Salmonella in the environment and in potential reservoirs is of considerable importance. Palavras-chave: Salmonella Heidelberg, pulsed-field gel electrophoresis, public health, foodborne Agência de fomento:Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Oswaldo Cruz Foundation and Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro – FAPERJ | |||||